PathHunter® U2OS c-KIT Functional Assay

The PathHunter® U2OS c-KIT Functional Assay measures c-KIT receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0853C3 Size: 2 vials

Biological Information
Background Information:	The PathHunter® U2OS c-KIT Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-KIT dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged c-KIT and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the cyno version of the protein.
Target Class:	Kinase
Family:	Receptor Tyrosine Kinase
Accession Number:	NM_000222.2
Target Name:	c-Kit
Target Aliases:	v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Short term (24 h): Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Functional Activity
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®