PathHunter® Tocilizumab Bioassay Kit

Background Information:

The PathHunter® Tocilizumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-6) induced dimerization of IL-6ST and IL-6R. receptors. This product uses EFC technology. The cell line included overexpresses IL-6ST target protein fused to PK alongside IL-6R target protein fused to EA. Upon ligand-induced dimerization of IL-6ST and IL-6R, detectable EFC signal is produced. This assay has been optimized and qualified with Actemra (not supplied in the bioassay kit). Actemra is a registered trademark of Chugai Seiyaku Kabushiki Kaisha Corp., a member of the Roche Group. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Please reach out to Tech Support at DiscoverXSupportUS@discovery.eurofinsus.com for lot specific CoA.

Target Class:

Cytokine Receptor

Family:

IL-6 Receptor

Protein Name:

IL6RA

Protein Aliases:

IL-6R subunit alpha|IL-6R-alpha|IL-6RA|IL-6R 1|Membrane glycoprotein 80|gp80|CD antigen CD126

Accession Number:

NM_000565.3

UniProt Number:

P08887

Gene Name:

IL6R

Gene ID:

3570

Target Species:

Human

Cell Background:

U2OS