| Biological Information | |
|---|---|
| Background Information: | The PathHunter® Tocilizumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-6) induced dimerization of IL-6ST and IL-6R. receptors. This product uses EFC technology. The cell line included overexpresses IL-6ST target protein fused to PK alongside IL-6R target protein fused to EA. Upon ligand-induced dimerization of IL-6ST and IL-6R, detectable EFC signal is produced. This assay has been optimized and qualified with Actemra (not supplied in the bioassay kit). Actemra is a registered trademark of Chugai Seiyaku Kabushiki Kaisha Corp., a member of the Roche Group. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Cytokine Receptor |
| Family: | IL-6 Receptor Family |
| Accession Number: | NM_000565.3 |
| Target Name: | IL6R/IL6ST |
| Target Aliases: | interleukin 6 receptorIL6Qgp80CD126HIES5IL-6RIL6RAIL6RQIL-1RaIL-6RAIL6QTLIL-6R-1 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency, Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Short term (24 h): Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Dimerization |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | Actemra® |
| Trademark Statement: | Actemra® is a registered trademark of Chugai Seiyaku Kabushiki Kaisha Corp., a member of the Roche Group |
| Clinical Relevance | |
| Therapeutic Area: | Inflammation/Allergy |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® Tocilizumab Bioassay Kit
The PathHunter® Tocilizumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-6) induced dimerization of IL-6ST and IL-6R.
User Manuals & Protocols
70-346 PathHunter Tocilizumab Bioassay Kit REV2 User Manual
View DocumentDatasheets
93-1045B3x Datasheet
View Document