| Biological Information | |
|---|---|
| Background Information: | The PathHunter® Sargramostim Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses GM-CSF or Sargramostim-induced dimerization of CSF2RB and CSF2RA receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside CSF2RA target protein fused to EA. Upon ligand-induced dimerization of CSF2RB and CSF2RA, detectable EFC signal is produced. This assay has been optimized and qualified with Leukine® (not supplied in the bioassay kit). Leukine® is a registered trademark of Genzyme Corporation (licensed). Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Cytokine Receptor |
| Family: | Receptor Tyrosine Kinase |
| Accession Number: | NM_000395.2 |
| Target Name: | CSF2Rb/CSF2RA |
| Target Aliases: | CD131CDw131IL3RBIL5RBSMDP5betaGMRCD116CDw116CSF2RCSF2RAXCSF2RAYCSF2RXCSF2RYGM-CSF-R-alphaGMCSFRGMCSFR-alphaGMRGMR-alphaSMDP4alphaGMRCSF2RB protein |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency, Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Short term (24 h): Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Dimerization |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | Leukine® |
| Trademark Statement: | Leukine® is a registered trademark of Genzyme Corporation (licensed) |
| Clinical Relevance | |
| Therapeutic Area: | Inflammation/Allergy |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® Sargramostim Bioassay Kit
The PathHunter® Sargramostim Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses GM-CSF or Sargramostim-induced dimerization of CSF2RB and CSF2RA receptors.
User Manuals & Protocols
70-398 PathHunter Sargramostim Bioassay Kit User Manual REV0
View DocumentDatasheets
93-1078Y3x Datasheet
View Document