PathHunter® RXFP3 Total GPCR Internalization U2OS Cell Line

The PathHunter® RXFP3 Total GPCR Internalization U2OS Cell Line measures RXFP3 (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0948C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® RXFP3 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. relaxin-3) based internalization of RXFP3 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged RXFP3 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the RXFP3 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Relaxin Family Peptide Receptors
Sub Family:
Class A
Coupling:
Gi/Go
Accession Number:
NM_016568.2
Target Name:
RXFP3 (SALPR)
Target Aliases:
Relaxin family peptide receptor 3, G-protein coupled receptor GPCR135, G-protein coupled receptor SALPR, GPCR135, RLN3 receptor 1, RLN3R1, RXFPR3, SALPR, g protein-coupled receptor SALPR, relaxin 3 receptor 1, relaxin family peptide receptor 3, relaxin-3 receptor 1, relaxin/insulin-like family peptide receptor 3, somatostatin and angiotensin-like peptide receptor, somatostatin- and angiotensin-like peptide receptor
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Internalization
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-260 PathHunter Total GPCR Internalization Assay REV6 User Manual

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Datasheets

93-0948C3 Datasheet

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