PathHunter® RXFP3 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® RXFP3 Total GPCR Internalization Cell Line is a stable, engineered cell line designed to study drug candidates (small molecules or biologics) that affect GPCR activation and internalization. This cell line enables the assessment of ligand-induced internalization of RXFP3, for example, in response to relaxin-3. The assay is based on Enzyme Fragment Complementation (EFC) technology. The cell line overexpresses a PK-tagged RXFP3 receptor and an Enzyme Acceptor (EA) tag localized to the plasma membrane, enabling complementation of the two β-galactosidase enzyme fragments (EA and PK). Upon ligand-induced receptor activation, RXFP3 undergoes internalization, disrupting EA/PK complementation and resulting in a loss of chemiluminescent signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Relaxin Family Peptide

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

RXFP3

Protein Aliases:

G protein-coupled receptor SALPR|G-protein coupled receptor GPCR135|Relaxin family peptide receptor 3|Somatostatin- and angiotensin-like peptide receptor

Accession Number:

NM_016568.2

UniProt Number:

Q9NSD7

Gene Name:

RXFP3

Gene ID:

51289

Gene Aliases:

RLN3R1|SALPR

Target Species:

Human

Cell Background:

U2OS