| Biological Information | |
|---|---|
| Background Information: | The PathHunter® PTH Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses PTH based activation of PTHR GPCR activity via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged PTHR and EA-tagged β-Arrestin-2. Activation of PTHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Parathyroid Hormone Receptors |
| Sub Family: | Class B |
| Accession Number: | AY449732 |
| Target Name: | PTHR |
| Target Aliases: | Parathyroid hormone receptor 1, PFE, EKNS, PTHR, PTHR1 |
| Target Species: | Human |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | β-Arrestin Recruitment |
| Detection Method: | Chemiluminescence |
| Clinical Relevance | |
| Therapeutic Area: | Metabolic Diseases |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® PTH Bioassay Kit
The PathHunter® PTH Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses PTH based activation of PTHR GPCR activity via detection of β-arrestin recruitment.
User Manuals & Protocols
70-348 PathHunter PTH Bioassay Kit REV1 User Manual
View DocumentDatasheets
93-0315Y2x Datasheet
View Document