Specifications
Documentation
Biological Information
Background Information:
The PathHunter® PTH Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses PTH based activation of PTHR GPCR activity via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged PTHR and EA-tagged β-Arrestin-2. Activation of PTHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Parathyroid Hormone
Sub Family:
Class B
Protein Name:
PTH1
Protein Aliases:
PTH/PTHr receptor|Parathyroid hormone 1 receptor|PTH1 receptor
Accession Number:
AY449732
UniProt Number:
Q03431
Gene Name:
PTH1R
Gene ID:
5745
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Bioassay Kits
Application:
Potency, Commercial Release & Stability
Kit Components:
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
PathHunter®