PathHunter® PTGFR Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® PTGFR Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. prostaglandin F2) based internalization of PTGFR via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged PTGFR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGFR receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Coupling:

Gq

Protein Name:

FP

Protein Aliases:

PGF2-alpha receptor|Prostanoid FP receptor

Accession Number:

NM_000959.3

UniProt Number:

P43088

Gene Name:

PTGFR

Gene ID:

5737

Gene Aliases:

FP

Target Species:

Human

Cell Background:

U2OS