PathHunter® PRLHR Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® PRLHR Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. prolactin releasing peptide) based internalization of PRLHR via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged PRLHR. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting PRLHR-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Prolactin-Releasing Peptide

Sub Family:

Class A

Coupling:

Gq

Protein Name:

PRP

Protein Aliases:

PrRPR|G-protein coupled receptor 10|hGR3

Accession Number:

NM_004248

UniProt Number:

P49683

Gene Name:

PRLHR

Gene ID:

2834

Gene Aliases:

PrRPR

Target Species:

Human

Cell Background:

U2OS