PathHunter® Pertuzumab Bioassay Kit

Background Information:

The PathHunter® Pertuzumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses heregulin-β2-induced dimerization of ErbB2 and ErbB3 receptors. This product uses EFC technology. The included cells overexpress ErbB2 target protein fused to PK alongside ErbB3 target protein fused to EA. Upon ligand-induced dimerization of ErbB2 and ErbB3, detectable EFC signal is produced. This assay has been optimized and qualified with Perjeta (not supplied in the bioassay kit). Perjeta is a registered trademark of Genentech, Inc. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Please reach out to Tech Support at DiscoverXSupportUS@discovery.eurofinsus.com for lot specific CoA.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

HER2

Protein Aliases:

neuro/glioblastoma derived oncogene homolog|human epidermal growth factor receptor 2

Accession Number:

NM_004448.2

UniProt Number:

P04626

Gene Name:

ERBB2

Gene ID:

2064

Gene Aliases:

NEU|HER-2|CD340|HER2

Target Species:

Human

Cell Background:

U2OS