| Biological Information | |
|---|---|
| Background Information: | The PathHunter® Pertuzumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses heregulin-β2-induced dimerization of ErbB2 and ErbB3 receptors. This product uses EFC technology. The included cells overexpress ErbB2 target protein fused to PK alongside ErbB3 target protein fused to EA. Upon ligand-induced dimerization of ErbB2 and ErbB3, detectable EFC signal is produced. This assay has been optimized and qualified with Perjeta (not supplied in the bioassay kit). Perjeta is a registered trademark of Genentech, Inc. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | Receptor Typosine Kinase (RTK): Growth Factor Receptor |
| Accession Number: | NM_004448.2 |
| Target Name: | ErbB2-ErbB3 |
| Target Aliases: | NEU, NGL, HER2, TKR1, CD340, HER-2, VSCN2, MLN 19, MLN-19, c-ERB2, c-ERB-2, HER-2/neu, p185(erbB2), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Dimerization |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | Perjeta® |
| Trademark Statement: | Perjeta® is a registered trademark of Genentech, Inc. |
| Clinical Relevance | |
| Therapeutic Area: | Oncology/Immuno-Oncology |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® Pertuzumab Bioassay Kit
The PathHunter® Pertuzumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses heregulin-β2-induced dimerization of ErbB2 and ErbB3 receptors.
View bioassay qualification data.
User Manuals & Protocols
70-384 PathHunter Pertuzumab Bioassay Kit REV0 User Manual
View DocumentDatasheets
93-1042Y3x Datasheet
View Document