| Biological Information | |
|---|---|
| Background Information: | The PathHunter® Panitumumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses EGF-induced dimerization of ErbB2 and EGFR receptors. This product uses EFC technology. The included cells overexpress ErbB2 target protein fused to PK alongside EGFR target protein fused to EA. Upon ligand-induced dimerization of ErbB2 and EGFR, detectable EFC signal is produced. This assay has been optimized and qualified with Vectibix® (not supplied in the bioassay kit). Vectibix® is a registered trademark of Amgen Inc. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | Receptor Typosine Kinase (RTK): Growth Factor Receptor |
| Sub Family: | Protein Tyrosine |
| Accession Number: | NM_005228.3 |
| Target Name: | EGFR/ErbB2 |
| Target Aliases: | Epidermal growth factor receptor, ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | SH2 Recruitment |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | Vectibix® |
| Trademark Statement: | Vectibix® is a registered trademark of Amgen Inc. |
| Clinical Relevance | |
| Therapeutic Area: | Oncology/Immuno-Oncology |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® Panitumumab Bioassay Kit
The PathHunter® Panitumumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses EGF-induced dimerization of ErbB2 and EGFR receptors.
View bioassay qualification data.
User Manuals & Protocols
70-383 PathHunter Panitumumab Bioassay Kit REV1 User Manual
View DocumentDatasheets
93-1051Y3x Datasheet
View Document