PathHunter® OPRL1 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® OPRL1 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. nociceptin/orphanin FQ) based internalization of OPRL1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged OPRL1 and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRL1 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Opioid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

NOP (ORL1)

Protein Aliases:

LC132 receptor-like|orphanin FQ receptor|kappa3-related opioid receptor|nociceptin/orphanin FQ receptor

Accession Number:

AY268428

UniProt Number:

P41146

Gene Name:

OPRL1

Gene ID:

4987

Gene Aliases:

NOCIR|ORL1|OOR|KOR-3|NOPr

Target Species:

Human

Cell Background:

U2OS