PathHunter® MTNR1A Total GPCR Internalization DLD1 Cell Line

Background Information:

The PathHunter® MTNR1A Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. melatonin) based internalization of MTNR1A via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged MTNR1A and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MTNR1A receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Melatonin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

MT1

Protein Aliases:

Mel1a receptor

Accession Number:

NM_005958

UniProt Number:

P48039

Gene Name:

MTNR1A

Gene ID:

4543

Gene Aliases:

MEL-1A-R

Target Species:

Human

Cell Background:

DLD1