PathHunter® LTB4R Total GPCR Internalization U2OS Cell Line

The PathHunter® LTB4R Total GPCR Internalization U2OS Cell Line measures LTB4R (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0810C3 Size: 2 vials
Inquire
Biological Information
Background Information:
The PathHunter® LTB4R Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. leukotriene B4) based internalization of LTB4R via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged LTB4R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the LTB4R receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Leukotriene Receptors
Sub Family:
Class A
Coupling:
Gi/Go & Gq
Accession Number:
AY322535
Target Name:
LTB4R
Target Aliases:
Leukotriene B4 receptor, BLT1, BLTR, CMKRL1, GPR16, LTBR1, P2RY7, P2Y7, LTB4R
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Internalization
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-260 PathHunter Total GPCR Internalization Assay REV6 User Manual

View Document

Datasheets

93-0810C3 Datasheet

View Document
AssayComplete™ Cell Plating 5 Reagent
93-0563R5A
100 mL

$339.00

AssayComplete™ Cell Detachment Reagent
92-0009
50 mL

$109.00

AssayComplete™ Freezing Reagent F3
92-5103FR
2 x 50 mL

$339.00

AssayComplete™ Thawing Reagent T3
92-4103TR
100 mL

$249.00

PathHunter® Detection Kit
93-0001
Inquire
AssayComplete™ Cell Culture Kit-103
92-3103G
Kit

$397.00