PathHunter® LTB4R Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® LTB4R Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. leukotriene B4) based internalization of LTB4R via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged LTB4R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the LTB4R receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Leukotriene

Sub Family:

Class A

Coupling:

Gi/Go & Gq

Protein Name:

BLT1 (LTB4)

Protein Aliases:

LTB4-R1|Chemoattractant receptor-like 1|G-protein coupled receptor 16|P2Y purinoceptor 7|P2Y7

Accession Number:

AY322535

UniProt Number:

Q15722

Gene Name:

LTB4R

Gene ID:

1241

Gene Aliases:

BLT|BLT1|BLTR|CMKRL1|GPR16|P2RY7

Target Species:

Human

Cell Background:

U2OS