PathHunter® Jurkat SIRPa Signaling Cell Line

The PathHunter® Jurkat SIRPa Signaling Cell Line measures SIRPa signal by recruitment of SH2 domain protein to the activated receptor. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1135C19 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® SIRPa Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. CD47) based activation of SIRPa receptors via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged SIRPa target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Checkpoint Receptor
Family:
IgSF (immune inhibitory Receptor)
Accession Number:
NM_001040022.1
Target Name:
SIRPa
Target Aliases:
SIRPA, Signal regulatory protein alpha, BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1
Target Species:
Human
Cell Background:
Jurkat
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Signaling
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-394 PathHunter Checkpoint Signaling Assay-IgSF_REV3

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Datasheets

93-1135C19

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