PathHunter® Jurkat CD28 Signaling Assay

The PathHunter® Jurkat CD28 Signaling Assay measures CD28 signal by recruitment of SH2 domain protein to the activated receptor. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1126C19 Size: 2 vials
Inquire
Biological Information
Background Information:
The PathHunter® CD28 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. CD80, CD86) based activation of CD28 receptors via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CD28 target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Checkpoint Receptor
Family:
IgSF (immune co-stimulatory Receptor)
Sub Family:
CD Molecules
Accession Number:
NM_006139.3
Target Name:
CD28
Target Aliases:
T-cell-specific surface glycoprotein CD28
Target Species:
Human
Cell Background:
Jurkat
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Signaling
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-394 PathHunter Checkpoint Signaling Assay-IgSF_REV3

View Document

Datasheets

93-1126C19

View Document
AssayComplete™ Cell Plating 0 Reagent
93-0563R0A
100 mL

$339.00

AssayComplete™ Thawing Reagent T6
92-4106TR
100 mL

$249.00

AssayComplete™ Cell Culture Kit-101
92-3101G
Kit

$397.00

PathHunter® Bioassay Detection Kit
93-0933
Inquire
AssayComplete™ Freezing Reagent F5
92-5105FR
2 x 50 mL

$339.00