| Biological Information | |
|---|---|
| Background Information: | The PathHunter® Insulin Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses insulin based activation of INSRb RTK receptors via detection of SH2 domain protein recruitment. The included cells overexpress ProLink-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This assay has been optimized and qualified with USP Insulin (not supplied in the bioassay kit). Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | Receptor Typosine Kinase (RTK): Growth Factor Receptor |
| Sub Family: | Protein Tyrosine |
| Accession Number: | NM_000208 |
| Target Name: | INSRb |
| Target Aliases: | Insulin receptor, isoform B, HHF5, CD220 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | SH2 Recruitment |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | USP Insulin |
| Clinical Relevance | |
| Therapeutic Area: | Metabolic Diseases |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® Insulin Bioassay Kit
The PathHunter® Insulin Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses insulin based activation of INSRb RTK receptors via detection of SH2 domain protein recruitment.
View bioassay qualification data.
User Manuals & Protocols
70-321 PathHunter Insulin Bioassay Kit REV4 User Manual
View DocumentDatasheets
93-0466Y3x Datasheet
View Document