PathHunter® IL-3 Signaling Reporter Cell Line (U937)

The PathHunter Signaling Pathway Reporter Assays are stable clonal cell lines that monitor the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology.

Catalog #: 93-1181C054 Size: 2 vials

Biological Information
Background Information:	The PathHunter Signaling Pathway Reporter Assays are stable clonal cell lines that monitor the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. Each stable cell line is engineered to express a synthetic DNA reporter construct, comprising a pathway-specific transcription factor response element that drives expression of ePL-tagged reporter protein. The activated signaling pathway induces expression of the ePL-tagged reporter protein, which is measured by addition of the detection reagent containing EA, resulting in complementation of the two enzyme fragments, and production of an active enzyme that hydrolyzes the substrate to generate a chemiluminescent signal.
Target Class:	Signaling Pathway
Family:	Cytokine
Accession Number:	NC_000005.10
Target Name:	IL-3
Target Aliases:	MCGF, MULTI-CSFIL3
Target Species:	Human
Cell Background:	U937

Usage
Product Type:	Stable Cell Lines
Application:	Signaling Reporters
Storage Conditions:	Short term (24 h): Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Signaling
Detection Method:	Chemiluminescence

Clinical Relevance
Therapeutic Area:	Inflammation/Allergy

Additional Information
Brand:	PathHunter®