PathHunter® HEK 293 Tie2 Functional Assay

Background Information:

The PathHunter® HEK 293 Tie2 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated Tie2 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged Tie2 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

TIE2

Protein Aliases:

Endothelial tyrosine kinase|Tunica interna endothelial cell kinase|Tyrosine kinase with Ig and EGF homology domains-2|Tyrosine-protein kinase receptor TEK|Tyrosine-protein kinase receptor TIE-2|hTIE2|p140 TEK|CD antigen CD202b

Accession Number:

NM_000459.4

UniProt Number:

Q02763

Gene Name:

TEK

Gene ID:

7010

Gene Aliases:

TIE2|TIE-2|VMCM1|CD202b

Target Species:

Human

Cell Background:

HEK293