Biological Information

Background Information:

The PathHunter® LXRβ-NCoR1 Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. oxysterols) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged LXRβ-NCoR1 NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of LXRβ-NCoR1, induced interaction with SRCP leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

NHR

Family:

Liver X Receptor-Like

Sub Family:

Non-Steroid

Accession Number:

NM_007121.4; NM_006311

Target Species:

Human

Cell Background:

HEK293

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Protein Interaction

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®