PathHunter® IGF-1 Bioassay Kit

The PathHunter® IGF-1 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. IGF1, IGF2, agonist antibody) based activation of IGF1R receptors via detection of SH2 domain protein recruitment.

Catalog #: 93-0505Y1-00069

$1,989.00$8,979.00


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Biological Information
Background Information:
The PathHunter® IGF-1 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. IGF1, IGF2, agonist antibody) based activation of IGF1R receptors via detection of SH2 domain protein recruitment. The included cells overexpress ProLink-tagged IGF1R target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.
Target Class:
Kinase
Family:
Receptor Typosine Kinase (RTK): Growth Factor Receptor
Sub Family:
Protein Tyrosine
Accession Number:
NM_000875
Target Name:
IGF1R
Target Aliases:
Insulin-like growth factor 1 receptor, IGFR, CD221, IGFIR, JTK13
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Bioassay Kits
Application:
Potency; Commercial Release & Stability
Kit Components:
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Functional Activity
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-379_PathHunter_IGF-1_User Manual_Rev2

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Datasheets

93-0505Y1x Datasheet

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