PathHunter® HEK 293 GRPR β-Arrestin Cell Line

The PathHunter® HEK 293 GRPR β-Arrestin Cell Line measures GRPR (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0229C1 Size: 2 vials
Inquire
Biological Information
Background Information:
The PathHunter® GRPR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. gastrin-releasing peptide) based activation of GRPR GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged GRPR and EA-tagged β-Arrestin 2. Activation of GRPR stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Bombesin Receptor
Sub Family:
Class A
Coupling:
Gq
Accession Number:
NM_005314
Target Name:
GRPR (BB2 receptor)
Target Aliases:
Gastrin releasing peptide receptor, BB2, BB2R, BRS2
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual

View Document

Datasheets

93-0229C1 Datasheet

View Document
AssayComplete™ Cell Culture Kit-105
92-3105G
Kit

$397.00

AssayComplete™ Cell Detachment Reagent
92-0009
50 mL

$109.00

PathHunter® Detection Kit
93-0001
Inquire
AssayComplete™ Thawing Reagent T1
92-4101TR
100 mL

$249.00

AssayComplete™ Cell Plating 4 Reagent
93-0563R4A
100 mL

$339.00

AssayComplete™ Freezing Reagent F1
92-5101FR
2 x 50 mL

$339.00