PathHunter® HEK 293 EDG3 β-Arrestin Cell Line

Background Information:

The PathHunter® EDG3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. S1P) based activation of EDG3 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged EDG3 and EA-tagged β-Arrestin 2. Activation of EDG3 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Cyno version of the protein.

Target Class:

GPCR

Family:

Lysophospholipid (S1P)

Sub Family:

Class A

Coupling:

Gq

Protein Name:

S1P3

Protein Aliases:

EDG3|Endothelial differentiation G-protein coupled receptor 3|Lysophospholipid receptor B3|Sphingosine 1-phosphate receptor Edg-3|S1P receptor Edg-3

Accession Number:

NM_005226

UniProt Number:

Q99500

Gene Name:

S1PR3

Gene ID:

1903

Gene Aliases:

EDG-3

Target Species:

Human

Cell Background:

HEK293