Biological Information

Background Information:

The PathHunter® cyno CXCR2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL8/IL8) based activation of CXCR2 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged cyno CXCR2 and EA-tagged β-Arrestin 2. Activation of CXCR2 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Cyno version of the protein.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CXCR2 (IL-8RB)

Protein Aliases:

CXCR-2|CDw128b|GRO/MGSA receptor|High affinity interleukin-8 receptor B|IL-8R B|IL-8 receptor type 2|CD antigen CD182

Accession Number:

EHH55156

UniProt Number:

G7PLE7

Gene Name:

CXCR2

Gene ID:

N/A

Target Species:

Cynomolgus Monkey

Cell Background:

HEK293

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®