PathHunter® GLP1R Activated GPCR Internalization U2OS Cell Line

The PathHunter® GLP1R Activated GPCR Internalization U2OS Cell Line measures GLP1R (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0724C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® GLP1R Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. exendin-4) based internalization of GLP1R via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged GLP1R. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting GLP1R-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Glucagon Receptor Family
Sub Family:	Class B
Coupling:	Gs
Accession Number:	NM_002062.3
Target Name:	GLP1R
Target Aliases:	Glucagon-like peptide receptor 1, GLP-1, GLP-1-R, GLP-1R
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	Internalization
Detection Method:	Chemiluminescence

Clinical Relevance
Therapeutic Area:	Metabolic Diseases

Additional Information
Brand:	PathHunter®