PathHunter® GCGR Total GPCR Internalization HEK 293 Cell Line

The PathHunter® GCGR Total GPCR Internalization HEK 293 Cell Line measures GCGR (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0820C1 Size: 2 vials

Inquire

Biological Information
Background Information:	The PathHunter® GCGR Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. glucagon) based internalization of GCGR via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged GCGR and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GCGR receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Glucagon Receptor Family
Sub Family:	Class B
Coupling:	Gs
Accession Number:	NM_000160
Target Name:	GCGR
Target Aliases:	Glucagon receptor, GGR, GL-R, MVAH
Target Species:	Human
Cell Background:	HEK293

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Internalization
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®