PathHunter® eXpress TORC1 Nuclear Translocation Assay

Background Information:

The PathHunter® eXpress TORC1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the CRTC/CREB pathway. This kit assesses ligand (e.g. forskolin) based pathway activation via detection of resulting TORC1 (CRTC1) nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged TORC1 (CRTC1) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Nuclear Protein

Family:

Transcription factor

Protein Name:

TORC1

Protein Aliases:

Transducer of regulated cAMP response element-binding protein 1|TORC-1|Transducer of CREB protein 1

Accession Number:

NM_015321.1

UniProt Number:

Q6UUV9

Gene Name:

CRTC1

Gene ID:

23373

Gene Aliases:

KIAA0616|MECT1|TORC1|WAMTP1

Target Species:

Human

Cell Background:

U2OS