PathHunter® eXpress SREBP2 Nuclear Translocation Assay

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The PathHunter® eXpress SREBP2 Nuclear Translocation Assay measures cholesterol biosynthesis pathway activation via nuclear translocation of SREBP2. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0830E3CP5L

$1,789.00 – $5,989.00

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Biological Information
Background Information:
The PathHunter® eXpress SREBP2 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the cholesterol biosynthesis pathway. This kit assesses ligand (e.g. 5'-UMP) based pathway activation via detection of resulting SREBP2 nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged SREBP2 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of SREBP2 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Nuclear Protein
Accession Number:
NM_004599.2
Target Name:
SREBP2
Target Aliases:
Sterol regulatory element binding transcription factor 2
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Translocation
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-300 PathHunter eXpress Nuclear Translocation Assays REV6 User Manual

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Datasheets

93-0830E3x Datasheet

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