The PathHunter® eXpress rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the rhesus version of the protein.
Receptor Tyrosine Kinase
Neurotrophic tyrosine kinase receptor type 1
eXpress Assay Kits
Drug Discovery & Development
Store in vapor phase of liquid nitrogen.
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
PathHunter® eXpress rhTrkA-rhP75 Functional Assay
The PathHunter® eXpress rhTrkA-rhP75 Functional Assay measures rhTrkA-rhP75 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. eXpress kits contain all assay materials: cells, reagents, and plates. Rhesus version.