Biological Information
Background Information:
The PathHunter® PTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged PTGER4 and EA-tagged β-Arrestin-2. Activation of PTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Prostanoid
Sub Family:
Class A
Coupling:
Gs
Protein Name:
EP4
Protein Aliases:
PGE2 receptor EP4 subtype|Prostanoid EP4 receptor
Accession Number:
AY429109
UniProt Number:
P35408
Gene Name:
PTGER4
Gene ID:
5734
Gene Aliases:
EP4|PTGER2
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®