Biological Information
Background Information:
The PathHunter® PRLR-JAK1 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses PRLR activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged PRLR cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Cytokine Receptor
Family:
Prolactin Receptor
Protein Name:
PRLR
Accession Number:
NM_000949
UniProt Number:
P16471
Gene Name:
PRLR
Gene ID:
5618
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
Assay Measures:
Functional Activity
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®