PathHunter® eXpress Keap1-NRF2 Nuclear Translocation Assay

The PathHunter® eXpress Keap1-NRF2 Nuclear Translocation Assay measures Keap1-NRF2 pathway activation via nuclear translocation of NRF2. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0821E3CP0L


Biological Information
Background Information:
The PathHunter® eXpress Keap1-NRF2 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the Keap1-NRF2 pathway. This kit assesses ligand (e.g. methyl CDDO) based pathway activation via detection of resulting NRF2 nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged NRF2 and nuclear localized EA alongside untagged Keap1. Upon pathway activation, induced nuclear translocation of NRF2 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Nuclear Protein
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Product Type:
eXpress Assay Kits
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
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Additional Information

User Manuals & Protocols

70-300 PathHunter eXpress Nuclear Translocation Assays REV6 User Manual

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93-0821E3x Datasheet

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