| Biological Information | |
|---|---|
| Background Information: | The PathHunter® IL1RL1/IL1RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-33) induced dimerization of IL1RL1/IL1RAP receptors. This product uses EFC technology. The cells included overexpress IL1RL1 target protein fused to PK alongside IL1RAP target protein fused to EA. Upon ligand-induced dimerization of IL1RL1/IL1RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | Cytokine Receptor |
| Family: | Immunoglobulin-like family of IL-1 Receptors |
| Accession Number: | NM_016232.4 |
| Target Name: | IL1RL1/IL1RAP |
| Target Aliases: | DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1, C3orf13, IL-1RAcP, IL1R3 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Dimerization |
| Detection Method: | Chemiluminescence |
| Clinical Relevance | |
| Therapeutic Area: | Inflammation/Allergy |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® eXpress IL1RL1/IL1RAP Dimerization Assay
The PathHunter® eXpress IL1RL1/IL1RAP Dimerization Assay measures ligand-induced dimerization of IL1RL1/IL1RAP . eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-301 PathHunter eXpress Dimerization Assay Kit REV3 User Manual
View DocumentDatasheets
93-1067E3x Datasheet
View Document