PathHunter® eXpress IL1R1/IL1RAP Dimerization Assay

Background Information:

The PathHunter® IL1R1/IL1RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-1α) induced dimerization of IL1R1/IL1RAP receptors. This product uses EFC technology. The cells included overexpress IL1RAP target protein fused to PK alongside IL1R1 target protein fused to EA. Upon ligand-induced dimerization of IL1R1/IL1RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Cytokine Receptor

Family:

IL-1 Receptor

Protein Name:

IL1R1

Protein Aliases:

IL-1RT-1|IL-1RT1|CD121 antigen-like family member A|Interleukin-1 receptor alpha|IL-1R-alpha|Interleukin-1 receptor type I|p80|CD antigen CD121a [Cleaved into: Interleukin-1 receptor type 1, membrane form|mIL-1R1|mIL-1RI; Interleukin-1 receptor type 1, soluble form|sIL-1R1|sIL-1RI]

Accession Number:

NM_000877.3

UniProt Number:

P14778

Gene Name:

IL1R1

Gene ID:

3554

Gene Aliases:

IL1R|IL1RA|IL1RT1

Target Species:

Human

Cell Background:

U2OS