PathHunter® eXpress HEK 293 RELA Nuclear Translocation Assay

The PathHunter® eXpress HEK 293 RELA Nuclear Translocation Assay measures NFκB pathway activation via nuclear translocation of RELA. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0907E1CP0L


Biological Information
Background Information:
The PathHunter® eXpress HEK 293 RELA Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the NFκB pathway. This kit assesses ligand (e.g. wortmannin) based pathway activation via detection of resulting RELA nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged RELA and nuclear localized EA. Upon pathway activation, induced nuclear translocation of RELA leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Nuclear Protein
Accession Number:
Target Name:
NF-κB (p65)
Target Species:
Cell Background:
Product Type:
eXpress Assay Kits
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Assay Measures:
Detection Method:
Additional Information

User Manuals & Protocols

70-300 PathHunter eXpress Nuclear Translocation Assays REV6 User Manual

View Document


93-0907E1x Datasheet

View Document