PathHunter® eXpress HEK 293 IkappaB Degradation Assay

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The PathHunter® eXpress HEK 293 IκB Degradation Assay measures pathway activation-induced degradation of IκB target protein. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0538E1CP7L

$1,789.00 – $5,989.00

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Biological Information
Background Information:
The PathHunter® eXpress HEK 293 IκB Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the NFκB pathway. This kit enables detection of IκB levels, allowing for assessment of NFκB pathway activation-induced IκB degradation. This product uses EFC technology. The cells included overexpress IκB target protein fused to PL. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of IκB EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Signaling Pathway
Accession Number:
NM_020529
Target Name:
IκB
Target Aliases:
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Degradation
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-278 PathHunter eXpress HEK-293 IkB Degradation Assay REV1 User Manual

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Datasheets

93-0538E1x Datasheet

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