PathHunter® eXpress HEK 293 IkappaB Degradation Assay

Background Information:

The PathHunter® eXpress HEK 293 IκB Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the NFκB pathway. This kit enables detection of IκB levels, allowing for assessment of NFκB pathway activation-induced IκB degradation. This product uses EFC technology. The cells included overexpress IκB target protein fused to PL. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of IκB EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Signaling Pathway

Protein Name:

IKBA

Protein Aliases:

IkB-alpha|IkappaBalpha|Major histocompatibility complex enhancer-binding protein MAD3

Accession Number:

NM_020529

UniProt Number:

P25963

Gene Name:

NFKBIA

Gene ID:

4792

Gene Aliases:

IKBA|MAD3|NFKBI

Target Species:

Human

Cell Background:

HEK293