PathHunter® eXpress HCRTR1 Total GPCR Internalization Assay

Background Information:

The PathHunter® HCRTR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. orexin A) based internalization of HCRTR1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged HCRTR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Orexin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

OX1

Protein Aliases:

orexin receptor 1

Accession Number:

AF041245

UniProt Number:

O43613

Gene Name:

HCRTR1

Gene ID:

3061

Gene Aliases:

OX1R

Target Species:

Human

Cell Background:

U2OS