PathHunter® eXpress GLP1R Total GPCR Internalization Assay

The PathHunter® eXpress GLP1R Total GPCR Internalization Assay measures GLP1R (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-1075E3CP5L

$1,789.00$5,989.00


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Biological Information
Background Information:
The PathHunter® GLP1R Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. exendin-4) based internalization of GLP1R via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged GLP1R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GLP1R receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Glucagon Receptor Family
Sub Family:
Class B
Coupling:
Gs
Accession Number:
NM_002062.3
Target Name:
GLP1R
Target Aliases:
Glucagon-like peptide receptor 1, GLP-1, GLP-1-R, GLP-1R
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Internalization
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-262 PathHunter eXpress Total GPCR Internalization Assays REV7 (1) User Manual

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Datasheets

93-1075E3x Datasheet

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