Biological Information
Background Information:
The PathHunter® GHSR1a (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. ghrelin) based activation of GHSR1a GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged GHSR1a and EA-tagged β-Arrestin-1. Activation of GHSR1a stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Ghrelin
Sub Family:
Class A
Coupling:
Gq
Protein Name:
GHSR
Protein Aliases:
GH-releasing peptide receptor|GHRP|Ghrelin receptor
Accession Number:
NM_198407
UniProt Number:
Q92847
Gene Name:
GHSR
Gene ID:
2693
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-1
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®