PathHunter® eXpress GALR1 Total GPCR Internalization Assay

Background Information:

The PathHunter® GALR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. galanin) based internalization of GALR1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged GALR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GALR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Galanin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

GAL1

Protein Aliases:

GALR-1

Accession Number:

AY541036

UniProt Number:

P47211

Gene Name:

GALR1

Gene ID:

2587

Target Species:

Human

Cell Background:

U2OS