PathHunter® eXpress FPRL1 Activated GPCR Internalization Assay

Background Information:

The PathHunter® FPRL1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. formylpeptides) based internalization of FPRL1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged FPRL1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting FPRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Formylpeptide

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

FPR2

Protein Aliases:

FMLP-R-I|Formyl peptide receptor-like 1|HM63|Lipoxin A4 receptor|LXA4 receptor|RFP

Accession Number:

AY225226

UniProt Number:

P25090

Gene Name:

FPR2

Gene ID:

2358

Gene Aliases:

LXA4R|HM63|FPRH2|FMLPX|FPR2A|FMLP-R-II|ALXR|FPR|FMLP

Target Species:

Human

Cell Background:

U2OS