PathHunter® eXpress FPR1 Total GPCR Internalization Assay

Background Information:

The PathHunter® FPR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. formylpeptides) based internalization of FPR1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged FPR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the FPR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Formylpeptide

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

FPR1

Protein Aliases:

N-formyl peptide receptor|FPR|N-formylpeptide chemoattractant receptor

Accession Number:

NM_002029

UniProt Number:

P21462

Gene Name:

FPR1

Gene ID:

2357

Gene Aliases:

FPR|FMLP

Target Species:

Human

Cell Background:

U2OS