PathHunter® eXpress FGFR4-β-Klotho Functional Assay

The PathHunter® eXpress FGFR4-β-Klotho Functional Assay measures FGFR4-β-Klotho receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-1060E3CP0L


Biological Information
Background Information:
The PathHunter® eXpress FGFR4-β-Klotho Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR4 and β-Klotho dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, β-Klotho Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Receptor Typosine Kinase (RTK): Growth Factor Receptor
Sub Family:
Protein Tyrosine
Accession Number:
Target Name:
Target Aliases:
Fibroblast growth factor receptor 4, TKF, JTK2, CD334
Target Species:
Cell Background:
Product Type:
eXpress Assay Kits
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Assay Measures:
Functional Activity
Detection Method:
Additional Information

User Manuals & Protocols

70-270 PathHunter eXpress Receptor Tyrosine Kinase Functional Assay Kit REV9 User Manual

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93-1060E3x Datasheet

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