PathHunter® eXpress F2RL1 Activated GPCR Internalization Assay

The PathHunter® eXpress F2RL1 Activated GPCR Internalization Assay measures F2RL1 (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0747E3CP5L


Biological Information
Background Information:
The PathHunter® F2RL1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. serine proteases, SLIGRL) based internalization of F2RL1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged F2RL1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting F2RL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Proteinase-Activated Receptors
Gi/Go & Gq
Accession Number:
Target Name:
F2RL1 (PAR2,GPR11)
Target Aliases:
Coagulation factor II (thrombin) receptor-like 1 (PAR2), F2RL1, PAR2, Proteinase-Activated, GPR11, PAR2
Target Species:
Cell Background:
Product Type:
eXpress Assay Kits
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Assay Measures:
Detection Method:
Additional Information

User Manuals & Protocols

70-261 PathHunter eXpress Activated GPCR Internalization Assays REV7 User Manual

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93-0747E3x Datasheet

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