PathHunter® eXpress cyno CXCR1 HEK 293 β-Arrestin GPCR Assay

Background Information:

The PathHunter® cyno CXCR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of cyno CXCR1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged cyno CXCR1 and EA-tagged β-Arrestin-2. Activation of cyno CXCR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CXCR1 (IL-8RA)

Protein Aliases:

CXCR-1|CDw128a|High affinity interleukin-8 receptor A|IL-8R A|IL-8 receptor type 1|CD antigen CD181

Accession Number:

EHH55157

UniProt Number:

G7PLE8

Gene Name:

CXCR1

Gene ID:

n/a

Gene Aliases:

CKR-1|CDw128a|CD181

Target Species:

Cynomolgus Monkey

Cell Background:

HEK293