PathHunter® eXpress cINSRb Functional Assay

Background Information:

The PathHunter® eXpress cINSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated cINSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged cINSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cyno version of the protein.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

IR

Protein Aliases:

Insulin receptor subunit alpha|INSRa

Accession Number:

XM_005587740.1

UniProt Number:

N/A

Gene Name:

INSR

Gene ID:

102139081

Gene Aliases:

CD220

Target Species:

Cynomolgus Monkey

Cell Background:

U2OS